基于蛋白质组学技术的鹿茸不同部位差异蛋白筛选

doi:10.11843/j.issn.0366-6964.2017.04.004

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (4): 618-626.doi: 10.11843/j.issn.0366-6964.2017.04.004

基于蛋白质组学技术的鹿茸不同部位差异蛋白筛选

刘华淼, 鞠妍, 张然然, 邢秀梅*

中国农业科学院特产研究所, 特种经济动物分子生物重点实验室, 长春 130112

收稿日期:2016-10-25 出版日期:2017-04-23 发布日期:2017-04-23
通讯作者: 邢秀梅，博士，研究员，博士生导师，主要从事特种经济动物种质资源保护与遗传育种研究，E-mail：xingxiumei2004@126.com
作者简介:刘华淼（1988-），男，山东德州人，硕士，主要从事特种经济动物种质资源保护与遗传育种研究，E-mail：liuhuamiao2000@163.com
基金资助:
特种动物遗传资源创新团队（CAAS-ASTIP-201X-ISAPS）

Differentially Expressed Proteins Screening in Different Parts of Sika Deer Velvet Antler by Comparative Proteomics

LIU Hua-miao, JU Yan, ZHANG Ran-ran, XING Xiu-mei*

State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China

Received:2016-10-25 Online:2017-04-23 Published:2017-04-23

摘要/Abstract

摘要：

旨在了解梅花鹿鹿茸不同部位蛋白表达差异，本研究以腊片部位、血片部位和骨片部位的梅花鹿鹿茸组织为试验材料，运用双向电泳（2-DE）和MALDI-TOF-TOF串联质谱技术对不同部位梅花鹿鹿茸差异表达蛋白质进行鉴定，并对差异蛋白进行生物信息学分析。结果表明，成功筛选出37个差异表达蛋白，主要涉及多细胞生物体发生、解毒、细胞成分组织或生物发生、细胞聚集、定位、对刺激的反应、生物黏附、发育、单一生物体发生、免疫系统等生物学过程。其中鹿茸腊片、血片、骨片组织中分别有18、5与14个蛋白质表达上调。对差异表达蛋白作进一步分析发现，转录延伸因子（EFB1）、视黄酸结合蛋白（CRABP1）在鹿茸的快速生长中起重要调控作用，而载脂蛋白A1（APOA1）、脂肪酸结合蛋白4（FABP4）、转甲状腺素蛋白（TTR）在鹿茸的快速骨化中起重要的调控作用，对鹿茸快速生长与骨化机制的进一步研究具有重要意义。

Abstract:

To screen the differentially expressed proteins in different parts of sika deer velvet antler，the parts of sika deer velvet antlers of Lapian, Xuepian and Gupian were selected as experimental material.The differentially expressed proteins were identified by two-dimensional gel electrophoresis (2-DE) and MALDI-TOF-TOF-MS, and analyzed using bioinformatics methods. The result showed that 37 differentially expressed proteins were detected, which were involved in the biological process of multicellular organismal process, detoxification, cellular component organization or biogenesis, cell aggregation, localization, response to stimulus, biological adhesion, developmental process, single-organism process and immune system. There were 18, 5 and 14 up-regulated proteins in the Lapian, Xuepian, Gupian，respectively. We found that EFB1 and CRABP1 might play vital role in the process of velvet antler accelerated growth, and APOA1, FABP4, TTR might play vital role in the process of velvet antler ossification. Taken together, the result provide a basis for further research on the molecular mechanisms involved in the accelerated growth and ossification of deer velvet antler.

中图分类号:

S825.2

刘华淼, 鞠妍, 张然然, 邢秀梅. 基于蛋白质组学技术的鹿茸不同部位差异蛋白筛选[J]. 畜牧兽医学报, 2017, 48(4): 618-626.

LIU Hua-miao, JU Yan, ZHANG Ran-ran, XING Xiu-mei. Differentially Expressed Proteins Screening in Different Parts of Sika Deer Velvet Antler by Comparative Proteomics[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 618-626.

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基于蛋白质组学技术的鹿茸不同部位差异蛋白筛选

Differentially Expressed Proteins Screening in Different Parts of Sika Deer Velvet Antler by Comparative Proteomics

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